ADAPTIVE ENZYMES INDUCED BY INSOLUBLE SUBSTRATES

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Assay of Enzymes with Insoluble or Unknown Substrates: The Membrane-Bound Quinone Reductases as an Example

The conventional assay method for the majority of enzymes envisages a reaction between substrates in aqueous solution. A measurable concentration of product is accumulated over time. This paradigm has served well for the characterization of many enzymes. Variations of the method, often using chromogenic or fluorogenic substrates, have been developed and are widely used for purposes such as clin...

متن کامل

Cleavage of mispaired heteroduplex DNA substrates by numerous restriction enzymes.

The utility of restriction endonucleases as a tool in molecular biology is in large part due to the high degree of specificity with which they cleave well-characterized DNA recognition sequences. The specificity of restriction endonucleases is not absolute, yet many commonly used assays of biological phenomena and contemporary molecular biology techniques rely on the premise that restriction en...

متن کامل

Ultranicro Analysis of Enzymes and Substrates by Enzymatic Amplification Reactions

The principle and kinetics of enzymatic cycling are described. The enzymatic cycling reaction is catalyzed by a couple of enzymes and amplifies stoichiometrically a pair of substrates participating in the reaction. Many biological substances can be converted quantitatively to substrates that can be amplified and measured by cycling reactions. Three cyclings in routine use (NAD, NADP and CoA cyc...

متن کامل

Assignment of endogenous substrates to enzymes by global metabolite profiling.

Enzymes regulate biological processes through the conversion of specific substrates to products. Therefore, of fundamental interest for every enzyme is the elucidation of its natural substrates. Here, we describe a general strategy for identifying endogenous substrates of enzymes by untargeted liquid chromatography-mass spectrometry (LC-MS) analysis of tissue metabolomes from wild-type and enzy...

متن کامل

Catalytic Efficiency of Chitinase-D on Insoluble Chitinous Substrates Was Improved by Fusing Auxiliary Domains

Chitin is an abundant renewable polysaccharide, next only to cellulose. Chitinases are important for effective utilization of this biopolymer. Chitinase D from Serratia proteamaculans (SpChiD) is a single domain chitinase with both hydrolytic and transglycosylation (TG) activities. SpChiD had less of hydrolytic activity on insoluble polymeric chitin substrates due to the absence of auxiliary bi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1949

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.58.5.714-715.1949